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FrHESC is an intensive laboratory and lecture course designed for young independent scientists, physicians, and established investigators seeking comprehensive and sophisticated training in research strategies and state-of-the-art methods on cellular, molecular and genetic approaches for advancing the Frontiers in Human Embryonic Stem Cell research. The course provides hands-on laboratory exercises to teach the methods to maintain, characterize, and utilize human embryonic stem cells, as well as to learn of their clinical potentials through pre-clinical investigations, in accordance with NIH and HESC provider requirements. The course’s primary focus on HESC frontiers involves truly interdisciplinary and international approaches owing to the diversity of HESC lines currently eligible, as well as the wide sweeping studies that will likely benefit from HESC applications. As such, the first half of the course involves fundamental HESC cell and molecular biology, whereas the second half engages pioneers across the full spectrum of NIH’s purview.